str kdel neomycin Search Results


wd repeats aa 65 306 of coro1a coro1a wd1 5 egfp  (Addgene inc)
93
Addgene inc wd repeats aa 65 306 of coro1a coro1a wd1 5 egfp
Fig. 1 (a) Gpm6a and Rac1 co-immunoprecipitate with <t>Coro1a</t> from rat hippocampal lysates. Western blot of proteins co-immunoprecipi- tated from rat hippocampal lysates using anti-Coro1a antibody and probed with anti-Gpm6a, anti-Rac1 and anti-Coro1a antibodies. For this purpose, the membrane (different kDa areas) was cut and incubated with indicated antibodies. Non-immune rabbit serum was used as a control. Gpm6a and Rac1 are present in the anti-Coro1a immunoprecipitate. Bands representing Gpm6a are indicated by stars. (b) Coro1a co-localizes with Gpm6a in hippocampal neurons. Confocal image of hippocampal neurons (4 DIV) co-immunostained with anti- bodies against Gpm6a (red), Coro1a (green) and dendritic marker MAP2 (blue). A portion of Gpm6a-labeled spots co-localizes with Coro1a (arrowheads; insets 1 and 2). Scale bar, 20 lm.
Wd Repeats Aa 65 306 Of Coro1a Coro1a Wd1 5 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdh str kdel  (Addgene inc)
93
Addgene inc pcdh str kdel
Fig. 1 (a) Gpm6a and Rac1 co-immunoprecipitate with <t>Coro1a</t> from rat hippocampal lysates. Western blot of proteins co-immunoprecipi- tated from rat hippocampal lysates using anti-Coro1a antibody and probed with anti-Gpm6a, anti-Rac1 and anti-Coro1a antibodies. For this purpose, the membrane (different kDa areas) was cut and incubated with indicated antibodies. Non-immune rabbit serum was used as a control. Gpm6a and Rac1 are present in the anti-Coro1a immunoprecipitate. Bands representing Gpm6a are indicated by stars. (b) Coro1a co-localizes with Gpm6a in hippocampal neurons. Confocal image of hippocampal neurons (4 DIV) co-immunostained with anti- bodies against Gpm6a (red), Coro1a (green) and dendritic marker MAP2 (blue). A portion of Gpm6a-labeled spots co-localizes with Coro1a (arrowheads; insets 1 and 2). Scale bar, 20 lm.
Pcdh Str Kdel, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdh str kdel/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcdh str kdel - by Bioz Stars, 2026-05
93/100 stars
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Image Search Results


Fig. 1 (a) Gpm6a and Rac1 co-immunoprecipitate with Coro1a from rat hippocampal lysates. Western blot of proteins co-immunoprecipi- tated from rat hippocampal lysates using anti-Coro1a antibody and probed with anti-Gpm6a, anti-Rac1 and anti-Coro1a antibodies. For this purpose, the membrane (different kDa areas) was cut and incubated with indicated antibodies. Non-immune rabbit serum was used as a control. Gpm6a and Rac1 are present in the anti-Coro1a immunoprecipitate. Bands representing Gpm6a are indicated by stars. (b) Coro1a co-localizes with Gpm6a in hippocampal neurons. Confocal image of hippocampal neurons (4 DIV) co-immunostained with anti- bodies against Gpm6a (red), Coro1a (green) and dendritic marker MAP2 (blue). A portion of Gpm6a-labeled spots co-localizes with Coro1a (arrowheads; insets 1 and 2). Scale bar, 20 lm.

Journal: Journal of neurochemistry

Article Title: Neuronal filopodium formation induced by the membrane glycoprotein M6a (Gpm6a) is facilitated by coronin-1a, Rac1, and p21-activated kinase 1 (Pak1).

doi: 10.1111/jnc.13552

Figure Lengend Snippet: Fig. 1 (a) Gpm6a and Rac1 co-immunoprecipitate with Coro1a from rat hippocampal lysates. Western blot of proteins co-immunoprecipi- tated from rat hippocampal lysates using anti-Coro1a antibody and probed with anti-Gpm6a, anti-Rac1 and anti-Coro1a antibodies. For this purpose, the membrane (different kDa areas) was cut and incubated with indicated antibodies. Non-immune rabbit serum was used as a control. Gpm6a and Rac1 are present in the anti-Coro1a immunoprecipitate. Bands representing Gpm6a are indicated by stars. (b) Coro1a co-localizes with Gpm6a in hippocampal neurons. Confocal image of hippocampal neurons (4 DIV) co-immunostained with anti- bodies against Gpm6a (red), Coro1a (green) and dendritic marker MAP2 (blue). A portion of Gpm6a-labeled spots co-localizes with Coro1a (arrowheads; insets 1 and 2). Scale bar, 20 lm.

Article Snippet: Mammalian expression plasmids: pRFP-C1 encoding the red fluorescent protein (RFP), pEGFP-C1 (Clontech Laboratories, © 2016 International Society for Neurochemistry, J. Neurochem. (2016) 137, 46--61 Mountain View, CA, USA) encoding the enhanced green fluorescent protein (EGFP), RFP-tagged wild-type (wt) Gpm6a (Gpm6aRFP) described previously (Alfonso et al. 2005), EGFP-tagged wt Coro1a (wtCoro1a-EGFP), and a deletion mutant containing only 5 WD repeats (aa 65-306) of Coro1a [Coro1a(WD1-5)-EGFP] kindly provided by Dr William Trimble (Yan et al. 2005), EGFPtagged Rac1 T17N [Rac1DN-EGFP; Addgene#12982 (Subauste et al. 2000)], EGFP-tagged Rac1 Q61L [Rac1CA-EGFP; Addgene#12968 (Subauste et al. 2000)], myc-tagged Pak1 K299R [Addgene#12210 (Sells et al. 1997)], and myc-tagged Pak1 H83L/H86L [Addgene#12211 (Sells et al. 1997)]. siRNAs: siGENOME non-targeting siRNA Pool #2, Coro1a siRNAs targeting either its coding DNA sequence (CDS) [prevalidated in Suo et al. (Suo et al. 2014)] or its 30-UTR region (custom designed).

Techniques: Western Blot, Membrane, Incubation, Control, Marker, Labeling